Linearized form of Michaelis-Menten equation where both sides are put in reciprocal form and equated. 1/V= (Km/Vmax)(1/[s] + 1)Vmax where V is reaction velocity, [S] is substrate concentration, and Km is the Michaelis-Menten constant. Y-intercept allows calculation of Vmax and X-intercept allows calculation of Km.