A largely discarded method of detecting the hemoglobin content of blood. Uses a reference tube and a sample tube. The reference tube is made by taking known amount of blood from a normal person and combining this with a known amount of distilled water and preserving in a small sealed tube. A sample tube is graduated from 10 to 100%. The same amount of test blood is placed in the sample tube and is diluted with distilled water in increments until the tint is matched exactly to the tint in the reference tube. The corresponding percent mark is then read off and is the approximate amount of hemoglobin by percent that a person has in comparison to a normal person.